Branched-chain amino acid aminotransferase gene and use thereof

Details Branched-chain amino acid aminotransferase gene and use thereof Branched-chain amino acid aminotransferase gene and use thereof

C - Chemistry, Metallurgy

12

N

C12N 9/10 (2006.01) C07K 14/395 (2006.01)

Patent

CA 2602357

The present invention relates to a branched-chain amino acid aminotransferase gene and a use thereof, particularly, to a brewery yeast for producing alcoholic drinks with enhanced flavor, to alcoholic drinks produced with said yeast and to a method for producing said drinks. More particularly, the present invention relates to a yeast whose capacity of producing amyl alcohol and/or isobutanol and/or isoamyl acetate that contribute to the product flavor is controlled by controlling the expression levels of BAT1 and BAT2 genes coding for brewery yeast branched-chain amino acid aminotransferases Bat1p and Bat2p, respectively, particularly nonScBAT1 or nonScBAT2 gene specific to lager brewing yeast, and to a method for producing alcoholic drinks using said yeast.

L'invention concerne un gène d'aminotransférase d'acide aminé à chaîne ramifiée et son utilisation, plus précisément une levure de bière destinée à la production de boissons alcoolisées à arôme amélioré, des boissons alcoolisées produites à base de cette levure et un procédé de production de ces boissons. Plus précisément, l'invention concerne une levure dont la capacité de production d'alcool amylique et/ou d'isobutanol et/ou d'acétate d'isoamyle qui contribuent à l'arôme du produit, qui est régulée par régulation des niveaux d'expression des gènes BAT1 et BAT2 codant pour les Bat1p et Bat2p d'aminotransférases d'acide aminé à chaîne ramifiée de levure de bière, respectivement, en particulier le gène nonScBAT1 ou nonScBAT2 qui est spécifique aux levures de bières de fermentation basse. L'invention concerne enfin un procédé de production de boissons alcoolisées utilisant ladite levure.

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