Breast cancer proteins

Details Breast cancer proteins Breast cancer proteins

G - Physics

01

N

G01N 33/68 (2006.01)

Patent

CA 2581045

The present invention relates to a pooling method for performing proteomics with human samples, preferably derived from microdissected human samples, wherein the samples are grouped into two or more pools and each pool reveals at least one protein that is differentially manifest between these pools but common to a member of a given pool. Besides, the invention refers to proteins whose abundances are upregulated in cancer cells, especially in breast cancer cells, preferably in breast cancer cells that are positive or negative towards hormone binding receptors.

L'invention concerne un procédé de mise en commun permettant d'assurer l'analyse protéomique d'échantillons humains, de préférence dérivés d'échantillons humains microdisséqués, les échantillons étant regroupés dans au moins deux cuves, chaque cuve révélant au moins une protéine abondante de manière différentielle entre les cuves mais commune à un élément d'une cuve donnée. L'invention concerne en outre des protéines dont les abondances sont régulées à la hausse dans des cellules cancéreuses, en particulier dans des cellules du cancer du sein, et de préférence dans des cellules du cancer du sein positives ou négatives envers des récepteurs se liant à une hormone.

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