Bridging group to attach immunological substance to a latex

A - Human Necessities – 61 – K

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167/44

A61K 39/00 (2006.01) G01N 33/543 (2006.01)

Patent

CA 1089764

ABSTRACT OF THE DISCLOSURE A two-step process for achieving a covalent bond between latex polymers and immunological substances, the first of which involves reacting latex particles having a reactive carboxyl group with a diamine in the presence of an appropriate carbodiimide as the condensing agent. The resulting product, which can be designated as the modified latex, is an amide terminating in primary or secondary amino groups. This product is then reacted with the appropriate immunological reagent such as an antigen or antibody modified if necessary to contain amino groups, in the presence of a bifunctional aldehyde to result in the condensation of the amino groups of the immunological reagent to the amino groups of the modified latex via an intermediate aldehyde bridging moiety.

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