Bridging the sulphur atom of antigen, antibody or fragments...

Details Bridging the sulphur atom of antigen, antibody or fragments... Bridging the sulphur atom of antigen, antibody or fragments...

G - Physics

01

N

167/37, 530/15.0

G01N 33/543 (2006.01) A61K 39/44 (2006.01) C07D 263/44 (2006.01)

Patent

CA 1118344

ABSTRACT Insolublised antibodies, F(ab')2 fragments thereof and protein antigens are made by coupling to a water-insoluble substrate using a bridging agent which covalently binds to sulphur atoms in antibody, fragment or protein rather than to free amino groups therein. This is preferably effected by using a chloracetyl-terminated bridging agent. Immunoassays utilising these insolubilised reagents are described. One novel class of bridging agents comprising anhydride and monochloracetyl end groups, which is useful for making the said reagents, is also disclosed.

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