Broad host range dna cloning system for gram-negative bacteria

Details Broad host range dna cloning system for gram-negative bacteria Broad host range dna cloning system for gram-negative bacteria

C - Chemistry, Metallurgy

12

N

195/1.16, 195/1.

C12N 15/00 (2006.01) C12N 1/20 (2006.01)

Patent

CA 1192149

ABSTRACT OF THE DISCLOSURE Novel compositions and methods for cloning DNA in a broad range of Gram-negative bacteria. Relatively small vectors are prepared substantially free of DNA sequences coding for transfer functions, while having a broad host range replication function. While the plasmid is not self- transmissible, it is transmissible by means of a helper plasmid, which desirably has a narrow host range replication function. Exogenous DNA may be inserted into the first plasmid and conjugally transferred to the target host by means of a convenient bacterial host.

393703

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