Broad host range small plasmid rings as cloning vehicles

C - Chemistry – Metallurgy – 12 – N

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Details

195/1.18, 195/1.

C12N 15/00 (2006.01) C12N 15/52 (2006.01) C12N 15/74 (2006.01) C12N 15/78 (2006.01) C12P 13/06 (2006.01) C12P 13/08 (2006.01) C12P 13/12 (2006.01)

Patent

CA 1150169

ABSTRACT OF THE DISCLOSURE The present invention relates to novel, broad bacterial host range, small plasmid deoxyribonucleic acid rings which serve as cloning vehicles for DNA fragments, particularly those separated from other plasmid rings or from chromosomes, recombined with the small plasmid rings and to the processes for recombining the plasmid rings and to processes for transferring them between host bacteria. In particular, the present invention relates to the aggregate plasmid ring RPl/pRO1600, to pRO1600 and plasmid ring derivatives thereof, particularly including pRO1601; pRO1613 and pRO1614, all of which are carried for reference purposes in Pseudomonas aeruginosa ATCC 15692 (also known as strain PAOlc) and are on deposit at the Northern Regional Research Laboratories (NRRL) of the United States Department of Agriculture at Peoria, Illinois. The plasmid ring RPl (also known as R1822) is deposited in Pseudomonas aeruginosa NRRL-B-12123 (and is a known plasmid ring). The pRO1600 portion of the aggregate is a new plasmid ring. The novel small plasmid rings are particularly useful for recombinant genetic manipulation wherein the DNA fragments are introduced into the plasmid rings to produce useful, cloneable characteristics in the host bacterium, particularly chemical generating character- istics.

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