Broad-spectrum delta-endotoxins

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/32 (2006.01) A01H 5/00 (2006.01) A01N 63/02 (2006.01) A01P 7/04 (2006.01) C07K 14/325 (2006.01) C07K 16/12 (2006.01) C12N 15/82 (2006.01) C12P 21/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2272843

Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.

L'invention concerne de nouvelles protéines de cristal chimériques B.thuringiensis synthétiquement modifiées, présentant des propriétés insecticides améliorées contre les coléoptères, les diptères et les lépidoptères. L'invention concerne également les segments d'acide nucléique codant ces nouveaux peptides, ainsi que des procédés de production et d'utilisation de ces gènes et protéines et des procédés permettant l'expression recombinée et la transformation de cellules hôtes appropriées. Des cellules hôtes transformées et des plantes transgéniques exprimant l'endotoxine modifiée représentent aussi des aspects de la présente invention.

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