Broadly representative antigen sequences and method for...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/49 (2006.01) A61K 39/00 (2006.01) A61K 39/12 (2006.01) A61K 39/21 (2006.01) C07K 5/00 (2006.01) C07K 7/00 (2006.01) C07K 14/00 (2006.01) C07K 14/005 (2006.01) C07K 14/16 (2006.01) C12N 7/01 (2006.01) C12N 15/11 (2006.01) C12N 15/861 (2006.01) C40B 30/02 (2006.01) C40B 40/10 (2006.01) G01N 33/68 (2006.01) A61K 48/00 (2006.01) G06F 19/00 (2006.01)

Patent

CA 2681579

A novel method for generating vaccine sequences is disclosed herein that preserves contiguous epitope length stretches of amino acids or nucleotides from an input pool of sequences. The method generates continuous, stepwise epitope consensus that together provides for a single globally optimized sequence. The end sequences are designed to maximize overlap between any potential epitope length sequence extract from a natural antigen sequence. The disclosed method, thus, allows one to maximize the number of potential natural epitopes that are mimicked in a resultant vaccine sequence. Various representative HIV vaccine sequences have been generated and are disclosed herein.

La présente invention concerne un nouveau procédé consistant à générer des séquences de vaccins qui conserve les longueurs des déterminants antigéniques contigus d'acides aminés ou de nucléotides à partir d'un ensemble de départ de séquences. Le procédé génère les séquences consensus des déterminants antigéniques de manière continue et progressive qui pourvoient ensemble à l'établissement d'une unique séquence globalement optimisée. Les séquences finales sont conçues pour maximiser le chevauchement entre tout extrait de séquence de longueur maximale de déterminant antigénique potentiel à partir d'une séquence d'antigène naturel. Le procédé décrit permet ainsi de maximiser le nombre de déterminants antigéniques naturels potentiels qui sont copiés dans une séquence de vaccin résultante. Diverses séquences représentatives de vaccins contre le VIH ont été générées et sont décrites ici.

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