Buffered wash composition, insolubilizing composition, test...

Details Buffered wash composition, insolubilizing composition, test... Buffered wash composition, insolubilizing composition, test...

G - Physics

01

N

167/43, 150/15.1

G01N 33/573 (2006.01) G01N 33/531 (2006.01) G01N 33/537 (2006.01) G01N 33/58 (2006.01) G01N 33/76 (2006.01)

Patent

CA 2028175

-0- BUFFERED WASH COMPOSITION, INSOLUBILIZING COMPOSITION, TEST KITS AND METHOD OF USE Abstract of the Disclosure A buffered aqueous composition is useful simultaneously as a wash solution and a dye-providing composition in specific binding assays involving enzyme-labeled specific binding reagents. The wash composition includes a dye-providing composition, a buffer and an organic solvent having a certain molecular weight and water-solubility. Another useful composition includes a particulate substrate having avidin attached thereto, and a peroxidase reducing agent. Either composition can be provided in a diagnostic test kit, and can be used to detect a specific binding ligand in assays.

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