Buffering agents for biopharmaceutical formulations

A - Human Necessities – 61 – K

Patent

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Details

A61K 47/12 (2006.01) A61K 9/08 (2006.01) A61K 39/395 (2006.01) A61K 47/26 (2006.01) A61K 47/34 (2006.01)

Patent

CA 2649538

The invention provides a biopharmaceutical formulation including an aqueous solution having a propionate buffer with a pH from about 4.0 to about 6.0, at least one excipient and an effective amount of a therapeutic polypeptide. The propionate buffer can include a concentration selected from between about 1-50 mM, 2-30 mM, 3-20 mM, 4-10 mM and 5-8 mM. The therapeutic polypeptide included in a biopharmaceutical formulation of the invention can include an antibody, Fd, Fv, Fab, F(ab'), F(ab)2, F(ab')2, single chain Fv (scFv), chimeric antibodies, diabodies, triabodies, tetrabodies, minibody, peptibody, hormone, growth factor or cell signaling molecule. The invention also provides a method of preparing a biopharmaceutical formulation. The method includes combining an aqueous solution having a propionate buffer with a pH from about 4.0 to about 6.0 and at least one excipient with an effective amount of a therapeutic polypeptide.

La présente invention concerne une formulation biopharmaceutique comprenant une solution aqueuse qui comprend un tampon de propionate dont le pH va d'environ 4,0 à environ 6,0, au moins un excipient et une quantité efficace d'un polypeptide thérapeutique. Le tampon de propionate peut avoir une concentration comprise entre environ 1-50 mM, 2-30 mM, 3-20 mM, 4-10 mM et 5-8 mM. Le polypeptide thérapeutique compris dans la formulation biopharmaceutique de l'invention peut comprendre un anticorps, Fd, Fv, Fab, F(ab'), F(ab)2, F(ab')2, Fv simple chaîne (scFv), des anticorps chimères, des anticorps dimères, des anticorps trimères, des anticorps tétramères, un mini-anticorps, un petitde-anticorps, une hormone, un facteur de croissance ou une molécule de signalisation cellulaire. L'invention concerne également un procédé pour préparer une formulation biopharmaceutique qui consiste à combiner une solution aqueuse comprenant un tampon de propionate dont le pH va d'environ 4,0 à environ 6,0, et au moins un excipient, avec une quantité efficace d'un polypeptide thérapeutique.

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