Burkholderia rhizoxina micro-organisms, novel endosymbionts...

C - Chemistry – Metallurgy – 12 – N

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C12N 1/20 (2006.01) C12P 17/14 (2006.01) C12P 17/16 (2006.01)

Patent

CA 2610893

The invention relates to a method for producing rhizoxin and derivatives of rhizoxin. The aim of the invention is to produce novel micro-organisms that synthesise rhizoxin or derivatives of rhizoxin, and to provide a method for producing rhizoxin or derivatives thereof, without the disadvantages of prior art and in a simpler manner with a higher yield. To this end, bacterial symbionts of Rhizopus sp., in the form of Burkholderia Rhizoxina DSM 17360 or other Burkholderia Rhizoxina strains, are isolated, cultivated and used for the fermentative production of rhizoxin and/or derivatives of rhizoxin in a submerged culture, and the rhizoxin and/or the derivatives thereof are subsequently isolated from the culture supernatant.

L~invention concerne un procédé de production de rhizoxine, et de dérivés de rhizoxine. L~objectif de cette invention est de créer de nouveaux micro-organismes pouvant synthétiser la rhizoxine ou des dérivés de rhizoxine, ainsi qu~un procédé de production de rhizoxine ou de dérivés de rhizoxine, qui permette de supprimer les inconvénients de l~art antérieur, et de fournir de la rhizoxine et/ou des dérivés de rhizoxine de manière moins complexe, et avec un rendement plus élevé. A cet effet, le procédé selon l'invention consiste à isoler des symbiotes bactériens de Rhizopus sp. se présentant sous la forme de Burkholderia Rhizoxina DSM 17360, ou d~utres souches de Burkholderia Rhizoxina, à les mettre en culture immergée pour la production fermentative de rhizoxine et/ou de dérivés de rhizoxine, puis à isoler la rhizoxine et/ou les dérivés de rhizoxine dans le surnageant de culture.

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