Butinol i esterase

Details Butinol i esterase Butinol i esterase

C - Chemistry, Metallurgy

12

N

C12N 15/55 (2006.01) C12N 1/20 (2006.01) C12N 1/21 (2006.01) C12N 9/16 (2006.01) C12N 15/63 (2006.01) C12P 7/00 (2006.01) C12P 41/00 (2006.01)

Patent

CA 2419275

The invention relates to novel proteins exhibiting esterase activity, in particular butinol I esterase activity, obtained from Pseudomonas glumae and to nucleic acid sequences that code therefor, expression cassettes, vectors and recombinant microorganisms. The invention also relates to a method for producing said proteins and to the use thereof for the enzymatic, in particular, enantioselective ester hydrolysis or interesterification of organic esters.

La présente invention concerne des protéines d'un nouveau type, qui présentent une activité estérase, notamment une activité butinol I estérase, et sont issues de Pseudomonas glumae, ainsi que des séquences d'acides nucléiques codant celles-ci, des cassettes d'expression, des vecteurs et des micro-organismes de recombinaison. La présente invention concerne également un procédé pour produire ces protéines, ainsi que leur utilisation dans l'hydrolyse ou la transestérification enzymatique, notamment énantiosélective, d'esters organiques.

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