C-nucleoside derivatives and their use in the detection of...

C - Chemistry – Metallurgy – 07 – H

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C07H 19/14 (2006.01) C07H 19/06 (2006.01) C07H 19/23 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2215176

The invention concerns pyrrolo-[3,2-d]pyrimidine, pyrazolo-[4,3-d]pyrimidine and pyrimidine-furanosides i.e. so-called C-nucleosides of the general formulae I -- V (see formula I - V) or appropriate derivatives as well as processes for their production. The compounds are in particular suitable as substrates for RNA or DNA polymerases and can thus be incorporated into RNA or DNA oligonucleotides. Therefore the compounds are especially suitable for labelling and for detecting nucleic acids and for DNA sequencing.

L'invention concerne des pyrrolo-[3,2d]pyrimidine furanosides, des pyrazolo-[4,3-d]pyrimidine furanosides et des pyrimidine furanosides, c'est-à-dire des "C-nucléosides" de formule (I) - (V), ainsi que les dérivés correspondants de ces composés et un procédé de production desdits composés. Ces composés sont particulièrement appropriés pour servir de substrats destinés à des polymérases d'ARN et d'ADN, et peuvent donc être incorporés dans des oligonucléotides d'ARN et d'ADN. Ces composés sont donc particulièrement appropriés pour marquer et déceler des acides nucléiques et pour le séquençage d'ADN.

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