C-terminal modification of polypeptides

Details C-terminal modification of polypeptides C-terminal modification of polypeptides

C - Chemistry, Metallurgy

12

N

C12N 9/76 (2006.01) C12N 15/10 (2006.01) C12Q 1/37 (2006.01)

Patent

CA 2569707

The invention relates to a mutated trypsin comprising an amino acid substitution both at position K60 and D189, and at least one more amino acid substitution by histidine at position N143 or position E151. Such trypsin mutant has a preferred cleavage site comprising the amino acids Xaal -Xaaz His, wherein Xaal is L, Y or F and Xaa2 is R or K. The invention also relates to a man-made polypeptide comprising a target peptide and the above cleavage site as well as to a method of producing C-terminally modified target peptides by using this mutated trypsin.

Cette invention a pour objet une trypsine transformée, comprenant une substitution d~acide aminé en position K60 comme en position D189, et au moins une autre substitution d~acide aminé par de l~histidine en position N143 ou en position E151. Ce mutant de trypsine possède un site de reconnaissance préférable comprenant les acides aminés Xaal -Xaaz His, au sein duquel Xaal est L, Y ou F et Xaa2 est R ou K. Cette invention a également pour objet un polypeptide artificiel comprenant un peptide cible ainsi que le site de reconnaissance précité, et un procédé de production de peptides cibles à C-terminal modifié à l~aide de cette trypsine transformée.

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