C-terminal sequencing of peptides which may include proline

G - Physics – 01 – N

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G01N 33/68 (2006.01)

Patent

CA 2159980

A carboxy terminal protein sequencing process is disclosed which utilizes diphenyl phosphoroisothiocyanatidate and a heterocyclic amine to produce a thiohydantoin derivative of the C-terminal amino acid. The derivative is readily cleaved. The method is useful to sequence through all of the the 20 naturally occuring amino acids.

On décrit une méthode de séquençage de protéines en partant du carboxy terminal, qui fait appel à du diphényle phosphoroisothiocyanatidate et à une amine hétérocyclique, pour produire un dérivé thiohydantoïne de l'acide aminé C-terminal. Le dérivé est facilement scindé. La méthode est utile pour un séquençage portant sur l'ensemble des 20 acides aminés naturels.

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