Caged compound cleaving process

G - Physics – 01 – N

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Details

G01N 33/53 (2006.01) C12Q 1/66 (2006.01) G01N 33/531 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2453628

Caged compounds, i.e. synthetic chemical compounds whose chemical or biochemical activity is normally controlled by photolysis to release an active moiety to participate in a chemical or biochemical reaction, are subjected to electrical current instead of light to release the active moiety. The process is especially useful in chemiluminescent biochemical assays, where it is required to measure the light output in order to quantify the analyte under measurement, since avoids confusion between input energy to trigger the reaction, and light output for measurement purposes.

Des composés "bloqués", c'est-à-dire des composés chimiques synthétiques dont l'activité chimique ou biochimique est normalement contrôlée par la photolyse de manière à assurer la libération d'un groupe fonctionnel actif pour participer à une réaction chimique ou biochimique, sont soumis au courant électrique au lieu de la lumière; de cette manière, ils libèrent le groupe fonctionnel actif. Le procédé est particulièrement utile dans dosages biochimiques chimioluminescents, où il est nécessaire de mesurer la sortie de la lumière pour quantifier l'analyte pendant la mesure, de manière à éviter la confusion entre, d'une part, l'énergie d'entrée destinée à déclencher la réaction et, d'autre part, la sortie lumineuse à des fins de mesure.

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