C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/31 (2006.01) A61K 38/16 (2006.01) A61K 38/46 (2006.01) A61K 39/05 (2006.01) A61K 39/40 (2006.01) C07H 21/00 (2006.01) C07H 21/04 (2006.01) C07K 14/34 (2006.01) C07K 16/12 (2006.01) C07K 16/40 (2006.01) C12N 9/16 (2006.01) C12N 15/55 (2006.01) G01N 33/569 (2006.01) G01N 33/573 (2006.01) A61K 38/00 (2006.01) A61K 39/00 (2006.01)
Patent
CA 2242430
The invention relates to the cloning and sequencing of the DNA encoding a membrane-associated proteolipid from C. matruchotii that is capable of inducing in vitro calcium binding. A proteolipid was extracted from cultures of C. matruchotii and separated into a lipid component and three apoproteins having molecular weights of approximately 5.0, 5.5 and 7.5 kDa as determined by SDS-PAGE. The invention also includes the polyclonal and monoclonal antibodies directed against the membrane associated proteolipid and immunological assays developed with these antibodies.
L'invention concerne le clonage et le séquençage de l'ADN codant pour un protéolipide à liaison membranaire, issu de C. matruchotii, qui est apte à induire in vitro la fixation du calcium. Un protéolipide a été extrait de cultures de C. matruchotti et séparé pour donner un constituant lipidique et trois apoprotéines présentant des poids moléculaires d'environ 5,0, 5,5 et 7,5 kDa selon détermination par SDS-PAGE. L'invention concerne également les anticorps polyclonaux et monoclonaux dirigés contre ce protéolipide à liaison membranaire, ainsi que des dosages immunologiques mis au point avec ces anticorps.
Boyan Barbara D.
Dean David D.
Van Dijk Simon
Board Of Regents The University Of Texas System
Deeth Williams Wall Llp
LandOfFree
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