Calcium complexing dyes and their use in analytical...

C - Chemistry – Metallurgy – 09 – B

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C09B 23/14 (2006.01) C09B 29/00 (2006.01) C09B 29/36 (2006.01) C09B 57/06 (2006.01) C09B 57/10 (2006.01) C09B 69/00 (2006.01) G01N 33/00 (2006.01) G01N 33/84 (2006.01)

Patent

CA 1258672

-0- CALCIUM COMPLEXING DYES AND THEIR USE IN ANALYTICAL COMPOSITIONS, ELEMENTS AND METHODS Abstract of the Disclosure Novel chromogenic derivatives of 1,2-bis- (o-aminoaryloxy)ethane-N,N,N',N'-tetraacetic acid are useful for the determination of calcium ions in both solution and dry assays. These compounds comprise a dye moiety which is directly conjugated to the acetic acid substituted-nitrogen atom and which enables the compounds to exhibit maximum absorbance at a wave- length generally greater than 400 nm before complexa- tion. When the compounds are complexed with calcium ions, the absorbance shifts to a shorter wavelength.

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