C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/57 (2006.01) C12N 9/52 (2006.01) C12N 9/54 (2006.01)
Patent
CA 2200563
Novel calcium free subtilisin mutants are taught, in particular subtilisins which have been mutated to eliminate amino acids 75-83 and which retain enzymatic activity and stability. Recombinant methods for producing same and recombinant DNA encoding for such subtilisin mutants are also provided.
L'invention se rapporte à de nouveaux mutants de la subtilisine sans calcium, notamment à des subtilisines qui ont été mutées pour éliminer les acides aminés aux positions 75 à 83 et qui conservent une activité et une stabilité enzymatiques. L'invention se rapporte également à des procédés de recombinaison servant à produire ces subtilisines et à l'ADN de recombinaison codant ces mutants de subtilisine.
Alexander Patrick A.
Bryan Philip N.
Strausberg Susan L.
Sim & Mcburney
University Of Maryland
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