Calibrating and testing immunoassays to minimize interferences

Details Calibrating and testing immunoassays to minimize interferences Calibrating and testing immunoassays to minimize interferences

G - Physics

01

N

G01N 33/53 (2006.01) C12Q 1/28 (2006.01) G01N 33/543 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2165935

A method for reducing interferent bias such as hemoglobin bias in immunoassays using dried slide test elements featuring peroxidase and leuco dye as the labeling mechanism. The method features the steps of: a) calibrating an immunoassay of a target analyte by ascertaining the time at which the rate of change in density produced by a first set of the test elements for the target analyte on at least one liquid having a known amount of interferent of choice and a known amount of target analyte, crosses over or minimizes the difference compared to the rate of change in density produced by a second set of test elements substantially identical to the first set of test elements, on a liquid having the known amount of target analyte but a negligible amount of interferent of choice, and b) conducting an assay of a patient sample of unknown amount of the target analyte by reading the rate of change in density in a third set of the test elements substantially identical to the first and second sets, caused by the sample, at a time corresponding to the cross-over time ascertained in step a).

Affiliated with

Also associated with

Rating

Calibrating and testing immunoassays to minimize interferences does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Calibrating and testing immunoassays to minimize interferences, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Calibrating and testing immunoassays to minimize interferences will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1730270