Calibration method and apparatus for optical scanner

G - Physics – 01 – N

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G01N 37/00 (2006.01) G01N 15/10 (2006.01) G01N 21/27 (2006.01) G01J 3/28 (2006.01)

Patent

CA 2198853

A calibration method and device for a fluorescence spectrometer which uses fluorescence from a homogeneous solid state standard (45) as the source of calibration fluorescence and wherein the solid state standard (45) may be built into the optical scanner (48) and the calibration may be automatically performed as a routine step when using the optical scanner (48). A gold standard (41) establishes fluorescent units, and the fluorescence spectrometer is calibrated by reference to calibration standards (45), such as calibration rubies, which are themselves rated against the gold standard and built into the fluorescence spectrometer to provide an unchanging reference to the gold standard and by which simultaneous calibration of two or more channels of a multi-channel fluorescence spectrometer may be accomplished.

L'invention concerne un procédé et un appareil d'étalonnage pour un spectromètre à fluorescence, faisant appel à la fluorescence d'un élément solide homogène comme étalon (45). L'étalon solide (45) peut être intégré dans le scanneur optique (48) et l'étalonnage peut être réalisé automatiquement de manière régulière lorsque l'on utilise le scanneur optique (48). Un étalon en or (41) fournit les unités de fluorescence et le spectromètre à fluorescence est étalonné par rapport à des étalons (45), tels que des rubis, lesquels sont étalonnés par rapport à l'étalon en or. Ces rubis sont incorporés dans le spectromètre à fluorescence où ils constituent une référence invariable par rapport à l'étalon en or et permettent d'étalonner simultanément deux ou plusieurs canaux dans un spectromètre à fluorescence à canaux multiples.

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