C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/82 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01)
Patent
CA 2454557
The present invention provides predominantly callus-specific promoters obtainable from Brassica napus plants. According to the present invention there is provided aDNA fragment harbouring a callus specific promoter, said DNA fragment being present in clone pJB1178-21 or clone pJB1178-43, respectively deposited with the Centraal Bureau of Schimmelcultures (Baarn, the Netherlands) on 6 February 2001 under no. CBS 109271 and no. CBS 109273. The DNA fragment according to the present invention is further characterised in that it comprises the nucleotide sequence represented by SEQ ID NO: 1 or SEQ ID NO: 2 or the full length sequences as depicted in SEQ ID NO: 8 or SEQ ID NO: 9 respectively or parts thereof. Further, the invention comprises the homologue sequences inArabidopsis thaliana as depicted in SEQ ID NO: 10 and SEQ ID NO: 11, respectively.
L'invention concerne principalement des promoteurs spécifiques de cals pouvant être obtenus à partir de Brassica napus. L'invention a trait à un fragment d'ADN contenant un promoteur spécifique de cal, ledit fragment d'ADN étant présent dans le clone pJB1178-21 ou dans le clone pJB1178-43, respectivement déposés au <= Centraal Bureau of Schimmelcultures >= (Baarn, Pays-Bas) le 6 février 2001 sous les numéros CBS 109271 et CBS 109273. Le fragment d'ADN de l'invention est en outre caractérisé en ce qu'il comprend la séquence nucléotidique représentée par SEQ ID NO: 1 ou SEQ ID NO: 2, ou les séquences pleine longueur décrites dans SEQ ID NO: 8 ou SEQ ID NO: 9, respectivement, ou des parties de celles-ci. De plus, l'invention concerne des séquences homologues d'Arabidopsis thaliana décrites dans SEQ ID NO: 10 et SEQ ID NO: 11, respectivement.
Bade Jacob Bernardus
Custers Jerome Hubertina Henricus Victor
Fetherstonhaugh & Co.
Syngenta Limited
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