Cam-operated gradient former

G - Physics – 05 – D

Patent

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Details

G05D 11/02 (2006.01) B01D 57/02 (2006.01) G01N 27/447 (2006.01)

Patent

CA 2146492

Gradient gels for electrophoresis are produced by an apparatus in which a pair of syringes are mounted on a support rack together with cams arranged for synchronous rotation. Cam followers engaged by the cams are linked to the syringe barrels, with each cam defining a distinct spiral path translated by one of the cam followers into a preselected rate or rate profile of volumetric discharge from one of the syringes as the cam rotates. With the rate or rate profile established by one cam being distinct from the rate or rate profile established by the other, a gradient or other variation in composition is obtained when the syringe discharges are combined into a common stream and directed to the gel enclosure for solidification into a gel. The gradient or variation in composition is determined by the cam profiles alone, and is independent of the rate at which the cams are rotated, and of whether or not the rotation is performed in a steady manner. Theapparatus can be readily designed for manual operation with highly accurate and reproducible results.

La présente invention vise des gels en gradient pour l'électrophorèse, produits au moyen d'un appareil dans lequel une paire de seringues est montée sur un support, avec des cames organisées pour tourner selon un mouvement synchrone. Les suiveurs de cames sont reliés aux corps des seringues, chaque came définissant, en tournant, une spirale distincte autour du suiveur, en fonction du dosage ou du profil de dosage volumétrique préétabli pour chaque seringue. Ce dosage ou profil de dosage étant différent pour chacune, le gradient ou autre type de variation de composition est obtenu lorsque le contenu expulsé de chaque seringue est combiné en une seule veine puis acheminé dans le compartiment de gel pour solidification. Le gradient ou variation de composition est déterminé uniquement par le profil des cames et n'est pas fonction de la vitesse ni de l'uniformité de rotation de ces dernières. L'appareil peut être facilement transformé pour être utilisé manuellement et permet des dosages très précis et reproductibles.

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