Cancer-targeted viral vectors

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/861 (2006.01) A61K 48/00 (2006.01) A61P 35/00 (2006.01) C12N 7/01 (2006.01) C12N 15/34 (2006.01)

Patent

CA 2593684

The present invention relates to viral vectors that are targeted to cancer cells. The viral vectors of the invention are adenoviruses having a PEG-3 promoter driving the expression of the viral genes ElA and ElB. The PEG-3 promoter exhibits increased activity in malignant cells. Adenoviruses of the invention show increased replication in malignant cells, thereby producing a cytopathic effect. The viral vectors of the invention may comprise additional genes of interest, and/or may have altered capsid proteins that may enhance infection of and/or target infection to cancer cells. Additional cell types derived from diseased states in which the PEG-3 promoter is selectively active are also therapeutic targets of the viral vectors of the instant invention including those generating allergic, autoimmune and inflammatory responses.

La présente invention concerne des vecteurs viraux ciblant des cellules cancéreuses. Les vecteurs viraux selon l'invention sont des adénovirus ayant un promoteur de PEG-3 commandant l'expression des gènes viraux EIA et EIB. Le promoteur de PEG-3 présente une activité accrue dans des cellules malignes. Les adénovirus selon l'invention présentent une duplication accrue dans des cellules malignes, et créent ainsi un effet cytopathique. Les vecteurs viraux selon l'invention peuvent comporter d'autres gènes d'intérêt et/ou des protéines de capsides modifiées pouvant augmenter une infection et/ou cibler des infections de cellules cancéreuses. D'autres types de cellules dérivées d'états pathologique dans lesquels le promoteur de PEG-3 est sélectivement actif sont également des cibles thérapeutiques desdits vecteurs viraux, tels que ceux produisant des réponses allergiques, auto-immunitaires et inflammatoires.

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