Capillary electrophoresis detection

G - Physics – 01 – N

Patent

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G01N 27/447 (2006.01)

Patent

CA 2099511

Detecting light in a capillary electrophoresis optical system includes input and output windows in the outside surface of a capillary tube. The width of the windows is not substantially greater than the bore of the tube. A fiber optic input is spaced from the capillary tube so that the light is directed to the tube and is matched to the window width. The light output from the tube is matched to be received by a fiber optic output spaced from the capillary tube. The optical light envelop from the fiber input through the tube and to the fiber output is governed by the LaGrange Invariant. The matching is effected in accordance with the Numerical Aperture of the fiber optics.

Des fenêtres d'entrée et de sortie disposées sur la surface externe d'un tube capillaire permettent de déceler la lumière dans un système optique d'électrophorèse capillaire. La largeur des fenêtres n'est que légèrement supérieure à l'alésage du tube. Une entrée de fibre optique correspondant à la largeur de la fenêtre est placée à une certaine distance du tube capillaire afin que la lumière soit dirigée vers le tube. La lumière émise du tube est reçue par une sortie de fibre optique placée à une certaine distance du tube. L'enveloppe optique, qui part de l'entrée de fibre optique, longe le tube et aboutit à la sortie de fibre optique, est régie par un invariant de Lagrange. L'appariement est fait en fonction de l'ouverture numérique des fibres optiques.

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