Capillary electrophoresis using clear coated capillary tubes

G - Physics – 01 – N

Patent

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G01N 27/447 (2006.01)

Patent

CA 2678751

A capillary tube having a hard, optically clear external coating or cladding. In one embodiment, the external clear coating comprises hard-fluoropolymer. The hard- fluoropolymer coating bonds to the fused silica glass, providing higher strength and superior static fatigue performance resulting in vastly improved bending flexibility. The thin hard- fluoropolymer coating of capillaries provides higher initial tensile strength, longer lifetime (resistance to stress corrosion or static fatigue) and superior ability to transmit excitation light and emitted light directly through the coating for fluorescence based detection.

L'invention concerne un tube capillaire munis d'un revêtement ou placage externe dur et optiquement clair. Dans un mode de réalisation, le revêtement externe clair comprend un fluoropolymère dur. Le revêtement de fluoropolymère dur se lie au verre de silice fondue, conférant une résistance mécanique plus élevée et une performance de fatigue statique supérieure conduisant à une flexibilité au pliage grandement améliorée. Le revêtement de fluoropolymère dur et fin des capillaires confère une résistance plus élevée à la traction initiale, une durée de vie plus longue (résistance à la corrosion sous contrainte ou à la fatigue statique) et une capacité supérieure à transmettre la lumière d'excitation et la lumière émise directement à travers le revêtement pour détection à base de fluorescence.

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