G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 27/447 (2006.01) B01D 57/02 (2006.01) G01N 21/64 (2006.01)
Patent
CA 2289864
A sample is separated and separated components thereof are successively fed to a part to be detected. A laser beam of at least 600 nm from a laser beam source of an optical measuring part is applied to the part to be detected through a dichroic mirror and a lens, for making a fluorochrome bonded to the separated components absorb multiphotons, exciting the fluorochrome and making the same fluoresce. The optical measuring part captures the fluorescence so that photomultipliers detect fluorescence of not more than 510 nm in wavelength, fluorescence longer than 510 nm and not more than 560 nm in wavelength, fluorescence longer than 560 nm and not more than 580 nm in wavelength and fluorescence longer than 580 in wavelength respectively. Thus, a capillary electrophoretic apparatus can detect fluorescence from a fluorochrome bonded to samples as a label without influence by Raman scattering or Rayleigh scattering.
Fujiwake Hideshi
Hayashizaki Yoshihide
Nakamura Shin
Japan Science And Technology Corporation
Kirby Eades Gale Baker
Shimadzu Corporation
The Institute Of Physical And Chemical Research
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