Capillary flow method and apparatus for determination of...

Details Capillary flow method and apparatus for determination of... Capillary flow method and apparatus for determination of...

G - Physics

01

N

150/13

G01N 21/01 (2006.01) G01N 13/04 (2006.01) G01N 33/48 (2006.01)

Patent

CA 1068587

CAPILLARY FLOW METHOD AND APPARATUS FOR DETERMINATION OF CELL OSMOTIC FRAGILITY ABSTRACT OF THE DISCLOSURE A method and apparatus for the determination of blood cell osmotic fragility, wherein a solution of salt, such as sodium chloride, with an osmotic gradient decreasing with time is passed through a coiled capillary tube, positioned with its axis vertical so that the flow through the turns of the tube is substantially horizontal, and the flow follows Poiseuille's parabolic flow pat- tern. The blood sample is inserted in the coiled tube and, because of the flow pattern, the sale solution travels through the capil- lary tube much faster than the erythrocytes, so that the erythro- cytes are exposed to gradually decreasing osmolarity for hemolysis. Optical monitoring is carried out as the hemoglobin is rapidly re- moved from the flowing stream. Optical density is recorded against time, employing a 547 nm monitoring beam, thus providing a hemolysis curve.

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