Capsular polysaccharide adhesin antigen, preparation,...

C - Chemistry – Metallurgy – 12 – P

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C12P 19/04 (2006.01) A61K 39/085 (2006.01) C07K 16/12 (2006.01) C12Q 1/06 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1317288

ABSTRACT A substantially pure capsular exopolysaccharide adhesin of coagulase-negative staphylococcal strains, and a general method to prepare such adhesins, are described. Vaccines composed of such adhesins, and uses of such adhesins to produce polyclonal and monoclonal antibodies against such adhesins, are also disclosed. The adhesins are useful in coating polymeric medical materials to prevent colonization by coagulase-negative staphylococcal strains, and as a probe in selecting desirable polymeric medical materials. Such adhesin antibodies are useful in vivo to prevent infection by noso- comial coagulase-negative staphylococcal strains, in assays for the dection of such bacteria, in assays for the estimat- tion of such adhesins in complex mixtures, and as an affinity chromatography matrix.

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