Capsular polysaccharide solubilisation and combination vaccines

A - Human Necessities – 61 – K

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Details

A61K 39/385 (2006.01) A61K 39/095 (2006.01) A61K 39/102 (2006.01) A61K 39/116 (2006.01) A61P 31/04 (2006.01) A61P 37/04 (2006.01) C12P 19/04 (2006.01)

Patent

CA 2450203

Precipitated bacterial capsular polysaccharides can be efficiently re- solubilised using alcohols as solvents. The invention provides a process for purifying a bacterial capsular polysaccharide, comprising the steps of (a) precipitation of said polysaccharide, followed by (b) solubilisation of the precipitated polysaccharide using ethanol. CTAB can be used for step (a). The material obtained, preferably following hydrolysis and sizing, can be conjugated to a carrier protein and formulated as a vaccine. Also, in vaccines comprising saccharides from the serogroups A and C, the invention provides that the ratio (w/w) of MenA saccharide : MenC saccharide is >1.

La présente invention concerne des polysaccharides capsulaires d'origine bactérienne précipités pouvant être efficacement resolubilisés en utilisant des alcools comme solvants. L'invention propose un procédé permettant la purification d'un polysaccharide capsulaire d'origine bactérienne, comportant des étapes de (a) précipitation dudit polysaccharide, suivie (b) d'une solubilisation du polysaccharide précipité au moyen d'éthanol. On peut utiliser le bromure de cétyltriméthylammonium pour l'étape (a). La substance obtenue, de préférence après hydrolyse et calibrage, peut être conjuguée avec une protéine porteuse et formulée en tant qu'un vaccin. En outre, dans des vaccins comprenant des saccharides en provenance des sérogroupes A et C, l'invention présente un rapport pondéral saccharide MenA/saccharide MenC > 1.

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