G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/52 (2006.01) G01N 21/76 (2006.01) G01N 33/53 (2006.01) G01N 33/58 (2006.01)
Patent
CA 2182326
Improved assay methods utilizing stable, enzymatically triggered chemiluminescent 1,2-dioxetanes with improved water solubility are provided. Assays are performed by a capsule chemistry analytical assay method wherein fluid capsules comprising discrete aqueous segments containing either a chemiluminescent dioxetane reagent or an activating agent separated by an oil-based isolating fluid are flowed through a conduit, and subsequently reacted to produce light. The improvement comprises using a dioxetane substituted with two or more water-solubilizing groups disposed on the dioxetane structure to provide the chemiluminescence. Compositions comprising such a dioxetane, a non-polymeric surfactant enhancer and optionally a fluorescer, provide enhanced chemiluminescence and eliminate the problem of reagent carryover when used in assays performed on capsule chemistry analytical systems.
Adolfsen Robert H.
Akhavan-Tafti Hashem
de Silva Renuka
Schaap A. Paul
Board Of Governors Of Wayne State (the)
Corporation Bayer
Gowling Lafleur Henderson Llp
LandOfFree
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