Capsules containing transfected cells, method for preparing...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/10 (2006.01) A61K 39/00 (2006.01) A61K 48/00 (2006.01) G01N 33/50 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2572326

The invention concerns a capsule containing cells that are transiently transfected with a gene of interest and entrapped within a biocompatible polymer membrane, a method of preparing those capsules, a method for in vivo assessing an activity of the protein secreted by the gene of interest, which comprises administering to a multicellullar organism the capsule and detecting an activity, a pharmaceutical composition comprising such a capsule, and the use thereof for the administration of a protein of interest to a subject.

L'invention concerne une capsule contenant des cellules qui sont transitoirement transfectées avec un gène présentant un intérêt et emprisonnées à l'intérieur d'une membrane en polymère biocompatible, un procédé de préparation de ces capsules, un procédé servant à évaluer in vivo l'activité de la protéine secrétée par le gène présentant un intérêt, lequel procédé comprend d'administrer à un organisme multicellulaire la capsule et de détecter l'activité, une composition pharmaceutique comprenant une telle capsule et l'utilisation de celle-ci pour l'administration à un sujet d'une protéine présentant un intérêt.

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