Capture method and device

Details Capture method and device Capture method and device

C - Chemistry, Metallurgy

12

N

C12N 5/00 (2006.01) C12M 1/26 (2006.01) C12M 3/06 (2006.01) C12M 3/08 (2006.01) C12N 1/06 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01) C12S 3/20 (2006.01)

Patent

CA 2105963

- 24 - ABSTRACT A method of separating cell nuclei from cells comprises: treating a fluid containing whole cells so as to selectively lyse the cytoplasmic membrane together with a small proportion of the nuclear membranes but leaving a large proportion of the cell nuclei intact; applying the treated fluid to a membrane whereby a mesh of DNA from the lysed nuclei is formed on the surface and captures intact cell nuclei. A device for use in the method is described.

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