Carbohydrases from acidophilic streptomyces

C - Chemistry – Metallurgy – 12 – N

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195/123, 195/39

C12N 9/00 (2006.01) C12N 9/92 (2006.01)

Patent

CA 1131143

ABSTRACT Acidophilic and acidoduric streptomycetes strains have been found to produce carbohydrases. These Streptomyces effectively elabo- rate glucose isomerase under acid conditions typically unfavorable for growth of conventional glucose isomerase producing Streptomyces. Sterilization of the culture and production media may be avoided by selectively propagating newly discovered Streptomyces acidodurans under acidic conditions which will effectively eliminate contaminating micro- organisms. The Streptomyces acidodurane herein also have the ability to undergo cultivation and elaborate glucose isomerase over a relatively broad pH range. Constitutive streptomycetes strains have also been isolated. Glucose isomerases derived from these Streptomyces strains are particularly effective for isomerizing glucose syrups to fructose- containing syrups.

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