Carboxypeptidase a.sub.2 and process for preparing same

Details Carboxypeptidase a.sub.2 and process for preparing same Carboxypeptidase a.sub.2 and process for preparing same

C - Chemistry, Metallurgy

12

N

195/128, 195/39.

C12N 9/48 (2006.01)

Patent

CA 1165705

ABSTRACT OF THE DISCLOSURE A new species of carboxypeptidase A.gamma. and a process for preparation thereof are disclosed. The new substance has an amino-terminal sequence with the primary structural formula of: Asn-Tyr-Ala-Thr-Tyr-His-Thr-Leu-Glu-Glu-Ile-Tyr- Asp-Phe-Met-Asp-Ile-Leu-Val-Ala-Glu-His-Pro-Gln- Leu-, and has the following physiocochemical and enzymochemical properties: (a) state: needle crystals, (b) molecular weight: 38900 (according to gel permeation method), (c) constituent amino acids: As listed in Table 1, (d) metal contained: One Zn atom per molecule, (e) coefficient of sedimentation: 3.3, (f) isoelectric point: 4.3 (at an ionic strength of 0.3), (g) specific substrates: N.alpha.-carbobenzoxyglycyl-L-phenylalamine and casein, (h) Michaelis constant: 20 mM (N.alpha.-carbobenzoxyglycyl-L-phenylala- nine as the substrate), and (i) optimum pH: 7 to 8. The new species of carboxypeptidase A.gamma. is extracted from porcine pancreas. The process for preparing the new species of carboxypeptidase A.gamma. comprises adding mammalian duodenum or an extract of mammalian duodenum to the porcine pancreas, thereby performing activation.

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