Carotene synthase gene and uses therefor

Details Carotene synthase gene and uses therefor Carotene synthase gene and uses therefor

C - Chemistry, Metallurgy

12

N

C12N 15/61 (2006.01) A01H 5/00 (2006.01) A01H 13/00 (2006.01) A23L 1/28 (2006.01) C07H 21/00 (2006.01) C07K 16/40 (2006.01) C12N 1/21 (2006.01) C12N 9/00 (2006.01) C12N 9/02 (2006.01) C12N 9/10 (2006.01) C12N 9/90 (2006.01) C12N 15/52 (2006.01) C12N 15/53 (2006.01) C12N 15/54 (2006.01) C12N 15/74 (2006.01) C12N 15/82 (2006.01) C12P 1/04 (2006.01) C12P 7/64 (2006.01) C12P 23/00 (2006.01)

Patent

CA 2485969

Described herein is a novel three domain gene from Schizochytrium, denoted carotene synthase, that encodes a protein with three different enzymatic activities; phytoene dehydrogenase (PD), phytoene synthase (PS), and lycopene cyclase (LC). Also described is the isolated gene encoding the carotene synthase, homologues thereof, the enzyme encoded by such gene, biologically active portions and homologues thereof, recombinant nucleic acid molecules, microorganisms and plants that have been genetically modified to increase or decrease the action of such gene, and methods of producing carotenoids and derivatives thereof or methods of producing microorganisms and lipid products lacking pigmentation using the knowledge of the carotene synthase described herein.

La présente invention concerne un nouveau gène de Schizochytrium à trois domaines, appelé carotène synthase, qui code pour une protéine présentant trois activités enzymatiques différentes; la phytoène déshydrogénase (PD), la phytoène synthase (PS) et la lycopène cyclase (LC). L'invention concerne également le gène isolé codant pour la carotène synthase, des homologues de cette dernière, l'enzyme codée par ledit gène, des parties biologiquement actives et des homologues de ce dernier, des molécules d'acide nucléique de recombinaison, des microorganismes et des plantes qui ont été génétiquement modifiés afin d'augmenter ou de diminuer l'action dudit gène, ainsi que des procédés de production de caroténoïdes et de dérivés de ces derniers ou des procédés de production de microorganismes et de produits lipidiques à hypopigmentation faisant appel aux connaissances de la carotène synthase selon l'invention.

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