C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 23/00 (2006.01) C07H 21/04 (2006.01) C12N 1/16 (2006.01) C12N 1/18 (2006.01) C12N 9/02 (2006.01) C12N 15/74 (2006.01)
Patent
CA 2575244
A novel CrtZ carotenoid hydroxylase, isolated from Brevundimonas vesicularis DC263, is provided that is useful for the production of hydroxylated carotenoids. Additionally, a previously identified hypothetical protein from Novosphingobium aromaticivorans has found to have carotenoid hydroxylase activity. Both hydroxylase genes exhibit low homology in comparison to other CrtZ hydroxylases previously reported. Expression of the hydroxylases in heterologous host cells enabled production of hydroxylated carotenoids.
L'invention concerne une enzyme caroténoïde hydroxylase CrtZ, isolée à partir de Brevundimonas vesicularis DC263, utile pour la production de caroténoïdes hydroxylées. Par ailleurs, on décrit une protéine hypothétique précédemment identifiée à partir de Novosphingobium aromaticivorans, ayant démontré une activité de caroténoïde hydroxylase. Les deux gènes hydroxylase ont une faible homologie par rapport à d'autres hydroxylases CrtZ précédemment identifiées. L'expression des hydroxylases dans des cellules hôtes hétérologues a permis la production de caroténoïdes hydroxylées.
Cheng Qiong
Sedkova Natalia
Tao Luan
E.i. Du Pont de Nemours And Company
Torys Llp
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