Carotenoid ketolase gene

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/53 (2006.01) C12N 9/02 (2006.01) C12N 15/62 (2006.01) C12N 15/63 (2006.01) C12P 19/34 (2006.01) C12P 21/02 (2006.01) C12P 23/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2451142

A ketolase gene has been isolated from Rhodococcus erythropolis AN12 strain encoding a carotenoid modification enzyme of the carotenoid biosynthetic pathway. The gene and gene product are the first isolated from a Rhodococcus strain. Six conserved amino acid motifs have been identified as the characteristic of this type of ketolase enzymes. The gene and gene product of the present invention may be used in a variety of ways for the production of keto-carotenoid compounds in a variety of organisms.

On a isolé un gène de cétolase de souche de <i>Rhodococcus erythropolis AN12</i> codant pour une enzyme de modification de caroténoïde du trajet biosynthétique du caroténoïde. Ce gène et ce produit génique sont les premiers éléments isolés à partir d'une souche de <i>Rhodococcus </i>. On a identifié six motifs d'amino acide conservés sous forme de caractéristique de ce type d'enzymes cétolase. On peut utiliser le gène et le produit génique de cette invention dans diverses techniques de production de composés céto-carotènoïde dans divers organismes.

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