Carotenoid ketolase genes and gene products, production of...

C - Chemistry – Metallurgy – 12 – P

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C12P 23/00 (2006.01) C07H 21/04 (2006.01) C07K 14/00 (2006.01) C12N 1/20 (2006.01) C12N 9/00 (2006.01) C12N 9/02 (2006.01) C12N 15/00 (2006.01) C12P 7/26 (2006.01)

Patent

CA 2333281

A purified nucleic acid sequence which encodes for a protein having ketolase enzyme activity and has the nucleic acid sequence of SEQ ID NO: 1 or 3, or has a sequence which encodes the amino acid sequence of SEQ ID NO: 2 or 4, as well as vectors and host cells containing them. Methods of use of the nucleic acid sequences to produce ketocarotenoid in host cells and methods of use of the nucleic acid sequences to modify the production of carotenoids in a host cell are included.

L'invention concerne une séquence d'acide nucléique purifiée codant pour une protéine présentant une activité enzymatique cétolase et comprenant la séquence nucléotidique SEQ ID NO: 1 ou 3, ou la séquence codant pour la séquence d'acides aminés SEQ ID NO: 2 ou 4, ainsi que des vecteurs et des cellules hôtes contenant ces séquences. L'invention concerne en outre des procédés consistant à utiliser ces séquences nucléotidiques pour produire du cétocaroténoïde dans des cellules hôtes et des procédés consistant à utiliser des séquences nucléotidiques pour modifier la production de caroténoïdes dans une cellule hôte.

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