Cassette-based strategy for engineering multiple domain...

Details Cassette-based strategy for engineering multiple domain... Cassette-based strategy for engineering multiple domain...

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C07K 19/00 (2006.01) C12N 15/11 (2006.01)

Patent

CA 2470922

A method to aid in the efficient construction of fusion proteins involving multiple domains is provided in which a new expression vector is created to allow rapid generation of a library of cassettes. Cassettes have a standard vector structure based on four specific restriction endonuclease sites and using a subtle property of blunt or compatible cohesive end restriction enzymes, they can be fused in any order and number of times. Furthermore, the insertion of PCR products into our expression vector or the recombination of cassettes can be dramatically simplified by screening for the presence or absence of a detectable activity. The utility of this new strategy was demonstrated by the creation of basic cassettes for protein targeting to subcellular organelles and for protein purification using multiple affinity tags.

Affiliated with

Also associated with

Rating

Cassette-based strategy for engineering multiple domain... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Cassette-based strategy for engineering multiple domain..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cassette-based strategy for engineering multiple domain... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1445874