Cassettes for the expression of storable proteins in plants

Details Cassettes for the expression of storable proteins in plants Cassettes for the expression of storable proteins in plants

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) C07K 16/00 (2006.01) C07K 16/16 (2006.01) C12N 15/13 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2246242

The invention relates to cassettes for the expression of storable gene products in leaves and specifically in seeds, especially single-chain antibody fragments in leaves and seeds of transgenic tobacco and pea plants. The fields of application of the invention are biotechnology, medicine (diagnosis and therapy), foodstuffs and plant control and agriculture. The expression cassette of the invention comprises constitutive or seed-specific promoters, the LeB4 signal peptide, a gene to be expressed and an ER retention signal. Preference is given to an expression cassette containing the CaMV 35S promoter as the constitutive promoter, the gene for a single-chain antibody fragment as the gene and the amino acid sequence KDEL as the ER retention signal.

L'invention concerne des cassettes pour l'expression de produits géniques, stables au stockage, dans des feuilles et, spécifiquement, dans des semences, en particulier de fragments d'anticorps à chaîne unique dans des feuilles et des semences de tabac et de pois transgéniques. L'invention couvre les domaines d'application tels que biotechnologie, médecine (diagnostic et thérapie), contrôle des denrées alimentaires et des végétaux, agriculture. La cassette d'expression selon l'invention comprend des promoteurs constitutifs ou spécifiques des semences, le peptide-signal LeB4, un gène à exprimer et un signal de rétention ER. On utilise de préférence une cassette d'expression renfermant comme promoteur constitutif, le promoteur CaMV 35S, comme gène, le gène pour un fragment d'anticorps à chaîne unique et, comme signal de rétention ER, la séquence aminoacide KDEL.

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