Cat-mouse heterohybridoma and gene fragment coding for...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/62 (2006.01) C07K 16/00 (2006.01) C07K 16/08 (2006.01) C07K 16/46 (2006.01) C12N 5/12 (2006.01) C12N 15/13 (2006.01)

Patent

CA 2022959

The present invention is directed to: a gene fragment which includes a DNA sequence coding for an amino acid sequence of a constant region of a feline immunoglobulin .lambda. chain; a gene fragment which includes a DNA sequence coding for an amino acid sequence of a constant region of feline immunoglubulin K chain; a gene fragment which includes a DNA sequence coding for the constant region of feline immunoglubulin .gamma. chain; a recombinant DNA molecule coding for an amino acid sequence of a mouse-cat chimeric antibody which includes a gene fragment coding for an amino acid sequence of a variable region of a mouse immunoglobulin and a gene fragment coding for an amino acid sequence of a constant region of a feline immunoglobulin wherein the latter gene fragment is linked to the 3' site of the former gene fragment; a polypeptide of a mouse-cat chimeric antibody which is expressed from a cell transformed with an expression vector for cells wherein the recombinant DNA molecule coding for an amino acid sequence of the mouse-cat chimeric antibody is incorporated; a cat-mouse heterohybridoma which produces feline immunoglobulin; and a process for preparing a feline immunoglobulin gene. These products and processes can be used for the diagnosis, treatment and prevention of feline diseases.

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