Catalyst transformation by a modified reaction intermediate...

Details Catalyst transformation by a modified reaction intermediate... Catalyst transformation by a modified reaction intermediate...

G - Physics

01

N

167/45

G01N 33/58 (2006.01) G01N 33/535 (2006.01) G01N 33/542 (2006.01) G01N 33/94 (2006.01)

Patent

CA 1304291

CATALYST TRANSFORMATION BY A MODIFIED REACTION INTERMEDIATE AS A MEANS FOR ENZYME IMMUNOASSAY ABSTRACT OF THE DISCLOSURE The invention provides a test composition for an immunoassay which includes an enzyme capable of initiating a signal generating process. The activity of the enzyme is modified when an activating group is covalently bonded thereto. A conjugate including a reaction intermediate or a reaction intermediate analog capable of supplying the activating group for the enzyme and the analyte is provided, along with a specific binding partner for the analyze, and reagent means responsive to the condition of the enzyme for generating a detectable signal in proportion to the amount of enzyme activated. The invention further provides a method for an immunoassay utilizing the test composition.

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