Catalyst transformation by protein phosphorylation as a...

Details Catalyst transformation by protein phosphorylation as a... Catalyst transformation by protein phosphorylation as a...

G - Physics

01

N

167/45

G01N 33/58 (2006.01) G01N 33/535 (2006.01) G01N 33/542 (2006.01) G01N 33/94 (2006.01)

Patent

CA 1304292

CATALYST TRANSFORMATION BY PROTEIN PHOSPHORYLATION AS A MEANS FOR ENZYME IMMUNOASSAY ABSTRACT OF THE DISCLOSURE A test composition for use in a specific binding enzyme immunoassay for detecting an analyte in a sample including: (a) a target protein capable of mediating a signal generating process, the mediating activity of the target protein altered by a regulatory group covalently bonded thereto; (b) a regulatory enzyme capable of catalyzing the covalent bonding of the regulatory group to the target protein; (c) a conjugate of a substrate molecule for the regulatory enzyme and an analyte, the substrate containing the regulatory group; (d) a specific binding partner for the analyte; and (e) reagent means for generating a detectable signal responsive to the condition of the target protein. An immunoassay method for determining the amount of analyte in a sample which method uses the above test material is also described.

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