Catalytic antibodies enantioselectively hydrolysing amino...

C - Chemistry – Metallurgy – 12 – P

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C12P 41/00 (2006.01) C07K 16/44 (2006.01) C12N 5/18 (2006.01) C12N 9/00 (2006.01) C12P 13/04 (2006.01)

Patent

CA 2190455

The present invention is directed to a process for conducting optical resolution of a racemic mixture of an amino acid derivatives and a process for preparing an optically active amino acid using a catalytic antibody enantioselectively hydrolyzing an amino acid ester derivative. The catalytic antibody and hybridoma producing said antibody are also provided. The hybridomas in the present invention are typically produced by stimulation with an antigen comprising as a hapten a compound of the formula: (see formula 3) wherein CBZ is N-benzyloxycarbonyl.

L'invention concerne la résolution de mélanges racémiques d'acides aminés pour obtenir des acides aminés optiquement actifs, faisant appel à un anticorps catalytique qui hydrolyse l'ester d'acide aminé d'une manière énantiosélective. L'invention concerne également cet anticorps catalytique et un hybridone produisant l'anticorps. De manière caractéristique, cet anticorps est produit en effectuant une stimulation antigénique avec le composé (3) comme haptène. Dans cette formule, CBZ représente un groupe N-benzyloxycarbonyle.

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