Catalytic nucleic acid-based diagnostic methods

Details Catalytic nucleic acid-based diagnostic methods Catalytic nucleic acid-based diagnostic methods

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2312288

This invention provides methods and kits useful for determining whether a subject is afflicted with a disorder characterized by the presence of one or more known nucleic acid mutations. The instant methods comprise steps of nucleic acid molecule isolation, amplification, contact with one or more catalytic nucleic acid molecules specifically cleaving a target sequence present either in the case of disorder or wild-type, but not both, and determining cleavage of the amplified segment(s).

L'invention porte sur des méthodes et trousses permettant de déterminer si un sujet souffre ou non de troubles caractérisés par la présence d'une ou plusieurs mutations connues d'un acide nucléique. Ces méthodes, instantanées, consistent: à isoler et amplifier des molécules d'acide nucléique, à les mettre en contact avec une ou plusieurs molécules d'acide nucléique catalyseur, ce qui coupe spécifiquement une séquence cible caractéristique d'un trouble ou d'un type sauvage (mais pas des deux), puis à identifier la coupure du ou des segments amplifiés.

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