Cationic complexes of polymer-modified adenovirus

C - Chemistry – Metallurgy – 12 – N

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C12N 15/86 (2006.01) A61K 47/48 (2006.01) A61K 48/00 (2006.01) C12N 15/861 (2006.01)

Patent

CA 2341516

An adenovirus complex including a complex of a cationic molecule and of an adenovirus having at least one polyalkalene glycol polymer bound thereto. The polyalkalene glycol polymer includes, but is not limited to, polyethylene glycol, methoxypolyethylene glycol, polymethylethylene glycol, polyhydroxypropylene glycol, polypropylene glycol, and polymethylpropylene glycol. Molecular weights for the polymer range from 200 to 20,000 Daltons, with 2000 to 12,000 Daltons being preferred. The adenovirus is a preferably recombinant adenoviral vector such as a recombinant viral vector containing a transgene. The polymer is bound, directly or indirectly, to the virus particle by covalent or noncovalent means. The cationic molecule is preferably a cationic polymer, such as DEAE-Dextran, or a cationic lipid. A composition containing the adenovirus complex and a carrier is also disclosed.

Un complexe d'adénovirus comprend un complexe d'une molécule cationique et d'un adénovirus auquel est lié au moins un polymère de polyalkylène glycol. Le polymère de polyalkylène glycol comprend, sans limitation aucune, le polyéthylène glycol, le méthoxypolyéthylène glycol, le polyméthyléthylène glycol, le polyhydroxypropylène glycol, le polypropylène glycol et le polyméthylpropylène glycol. Le poids moléculaire du polymère varie entre 200 et 20 000 daltons, la préférence se situant entre 2 000 et 12 000 Daltons. L'adénovirus est de préférence un vecteur adénoviral de recombinaison tel qu'un vecteur viral de recombinaison contenant un transgène. Le polymère est lié, directement ou indirectement, à la particule virale par un système covalent ou non covalent. La molécule cationique est de préférence un polymère cationique, tel que le DEAE-Dextran, ou un lipide cationique. L'invention concerne également une composition contenant le complexe d'adénovirus et un support.

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