Cationic lipid-nucleic acid complexes

A - Human Necessities – 61 – K

Patent

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Details

A61K 9/127 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2264140

The invention is related to a method for preparing an homogenous suspension of stable lipid-nucleic acid complexes or particles, comprising: a) combining one or more cationic lipids, one or more colipids, and one or more stabilizing additives to form a lipid suspension, b) combining the lipid suspension with a nucleic acid to form a complex or a particle, and optionally c) subjecting the complex or particle to a sizing procedure. It also concerns an homogenous suspension produced notably by the above method.

L'invention concerne un procédé permettant de préparer une suspension homogène de complexes ou de particules stables constitués de lipides et d'acides nucléiques. Selon ce procédé, a) on associe un ou plusieurs lipides cationiques, un ou plusieurs co-lipides et un ou plusieurs stabilisants, de façon à former une suspension lipidique; b) on associe la suspension lipidique à un acide nucléique, de façon à former un complexe ou une particule; et éventuellement c) on soumet le complexe ou la particule à un processus de calibrage. L'invention concerne également une suspension homogène produite notamment par le procédé ci-dessus.

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