Cationic polymer and lipoprotein-containing system for gene...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/87 (2006.01) A61K 31/70 (2006.01) A61K 47/42 (2006.01) A61K 47/48 (2006.01) A61K 48/00 (2006.01) C12N 15/11 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2259139

A composition for delivery of a selected nucleic acid into a targeted host cell comprises a complex of a hydrophobized, positively charged, biocompatible polymer; a lipoprotein; and a selected nucleic acid. Hydrophobic and electrostatic interactions between the components provide for the formation of the condensed DNA-containing complex. Preferred embodiments of the complex have a slightly positive surface charge and an average diameter of about 200 nm. Plasmids and oligonucleotides can be efficiently delivered with such compositions. A method of delivering a selected nucleic acid to a host cell is also disclosed.

L'invention concerne une composition permettant la fourniture d'un acide nucléique sélectionné à une cellule hôte ciblée. La composition comprend un complexe d'un polymère rendu hydrophobe, à charge positive, et biocompatible; une lipoprotéine; et un acide nucléique sélectionné. Des interactions hydrophobes et électrostatiques entre les composants permettent la formation du complexe condensé contenant de l'ADN. Dans des modes de réalisation préférés, le complexe comprend une charge superficielle légèrement positive ainsi qu'un diamètre moyen d'environ 200 nm. Des plasmides et des oligonucléotides peuvent être fournis de manière efficace avec de telles compositions. L'invention concerne également un procédé de fourniture d'un acide nucléique sélectionné à une cellule hôte.

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