Cdna clone for south african arbovirus no. 86

Details Cdna clone for south african arbovirus no. 86 Cdna clone for south african arbovirus no. 86

C - Chemistry, Metallurgy

12

N

C12N 15/40 (2006.01) A61K 39/12 (2006.01) C07K 14/18 (2006.01) C12N 7/01 (2006.01) C12N 7/04 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2221155

The present invention provides a recombinant DNA comprising a cDNA coding for an infectious South African Arbovirus No. 86 (S.A.AR86) virus RNA transcript and a heterologous promoter positioned upstream from the cDNA and operatively associated therewith. The present invention also provides an infectious RNA transcript encoded by the cDNA, and infectious attenuated viral particles produced from cells transfected with the RNA transcript encoded by the cDNA.

L'invention concerne un ADN de recombinaison comprenant un ADNc codant pour un produit de transcription de l'ARN d'un arbovirus infectieux d'Afrique du Sud N·86 (S.A.AR86) et un promoteur hétérologue placé en amont de l'ADNc et associé de manière fonctionnelle à ce dernier. L'invention se rapporte également à un produit de transcription de l'ARN infectieux codé par l'ADNc et des particules virales infectieuses atténuées produites à partir de cellules transfectées à l'aide du produit de transcription de l'ADN codé par l'ADNc.

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